Multi-Omics Analysis Reveals the Regulatory and Metabolic Mechanisms Underlying Low-Nitrogen Tolerance at the Flowering Stage in Rice

Crop productivity depends on nitrogen fertilization, but plants take up only an average of 30–50% the applied nitrogen. Developing rice cultivars with improved use efficiency or low-nitrogen (LN) tolerance is critical for sustainable agriculture. In this study, a backcross introgression line (G9) 15 introgressed regions from donor parent and its recurrent Shuhui 527 (G1), which are differentially responsive to LN stress, were used investigate mechanism mediating at flowering stage based metabolome transcriptome profiles. Three genes (LOC_Os02g40010, LOC_Os11g25260 LOC_Os11g47300) involved in purine metabolism, located regions, detected significantly higher expression levels G9 than G1 under contents two relative metabolites (uric acid guanine) different between genotypes. Additionally, (LOC_Os02g36880 LOC_Os08g05830) (3-phosphoglyceric sedoheptulose 7-phosphate) glycosis pentose phosphate pathway expressed G1. addition metabolism-related (OsLHT1 OsACR9) 23 mainly metabolism identified genotypes treatments. With comprehensive analysis transcriptomes metabolomes, our results reveal that active may be main factor contributing stage, also provide five new candidate improving during molecular breeding rice.